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Related Experiment Video

Updated: May 26, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

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Published on: November 11, 2022

Multiple component quantitative analysis for the pattern recognition and quality evaluation of Kalopanacis Cortex

Chu Van Men¹, Yu Seon Jang, Kwan Jun Lee

¹College of Pharmacy, Chungnam National University, Daejeon, 305-764, Korea.

Archives of Pharmacal Research

|January 3, 2012

Summary

High-performance liquid chromatography (HPLC) effectively evaluates Kalopanacis Cortex (KC) quality. Liriodendrin is identified as a key marker compound for quality control, distinguishing authentic KC from other species.

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Last Updated: May 26, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

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Published on: September 2, 2020

Area of Science:

Pharmacognosy
Analytical Chemistry
Phytochemistry

Background:

Kalopanacis Cortex (KC) is a traditional medicinal material with variable quality.
Standardized quality evaluation methods are crucial for ensuring the efficacy and safety of herbal medicines.

Purpose of the Study:

To develop and validate a quantitative and pattern recognition method for the quality evaluation of Kalopanacis Cortex (KC).
To identify suitable marker compounds for the quality control of KC.

Main Methods:

Quantitative analysis of four bioactive compounds (liriodendrin, pinoresinol O-β-D-glucopyranoside, acanthoside B, kalopanaxin B) using High-Performance Liquid Chromatography (HPLC).
Method validation including linearity, recovery, precision, and limit of detection.
Pattern recognition analysis using linear discriminant analysis (LDA) on thirty-two KC samples from four species.

Main Results:

The HPLC method demonstrated excellent linearity (r > 0.9995), recoveries (98.4% to 101.9%), and precision (RSD < 2.20).
Liriodendrin was identified as a suitable marker compound for KC quality control.
Linear discriminant analysis successfully discriminated authentic KC from inauthentic species, confirming the method's suitability for quality evaluation.

Conclusions:

The developed HPLC method is reliable for the quantitative analysis of liriodendrin in KC.
The study provides a robust approach for the quality control and authentication of Kalopanacis Cortex.