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Spectrophotometric detection of tyrosinase activity based on boronic acid-functionalized gold nanoparticles
Sa Li1, Langyong Mao, Yaping Tian
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
The Analyst
|January 5, 2012
Abstract:
A spectrophotometric method for the detection of tyrosinase activity is developed by utilizing the product-triggered aggregation of boronic acid-functionalized gold nanoparticles. Based on the changes of absorbance in UV-visible spectra, the assay shows extremely high sensitivity and lowered limit of detection of 1 × 10(-10) u mL(-1).
