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Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 26, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

ArrayMAPPIT: a screening platform for human protein interactome analysis.

Sam Lievens1, Nele Vanderroost, Dieter Defever

  • 1Department of Medical Protein Research, VIB and Department of Biochemistry, Ghent University, Ghent, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2012
PubMed
Summary
This summary is machine-generated.

Mammalian protein-protein interaction trap (MAPPIT) is a powerful technology for discovering protein interactions in human cells. A new ArrayMAPPIT assay enables high-density screening of numerous prey proteins for interaction partners.

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Last Updated: May 26, 2026

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Mammalian protein-protein interaction trap (MAPPIT) is a versatile two-hybrid technology.
  • It identifies and characterizes protein interactions within living mammalian cells.
  • The method relies on the complementation of a modified cytokine receptor complex.

Purpose of the Study:

  • To describe a novel protocol that enhances the utility of MAPPIT.
  • To increase MAPPIT's capability for identifying new protein interactions.
  • To present the ArrayMAPPIT assay for efficient screening.

Main Methods:

  • The ArrayMAPPIT assay utilizes high-density microtiter plates for prey protein arraying.
  • Reverse transfection is employed to introduce prey proteins into a bait-expressing cell pool.
  • Protein interactions are monitored through the activation of a reporter gene upon receptor complex complementation.

Main Results:

  • The ArrayMAPPIT assay allows for efficient screening of numerous prey proteins.
  • This method facilitates the identification of novel protein interaction partners.
  • The protocol increases the overall utility of MAPPIT for interaction studies.

Conclusions:

  • The ArrayMAPPIT assay represents a significant advancement in MAPPIT technology.
  • This high-density screening approach accelerates the discovery of protein interactions.
  • The developed protocol enhances the identification of novel protein interactions in mammalian cells.