An alternative method for cultivation of Lawsonia intracellularis
Fabio A Vannucci1, Suphot Wattanaphansak, Connie J Gebhart
1Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA. vannu008@umn.edu
Journal of Clinical Microbiology
|January 6, 2012
Abstract:
An alternative method for the cultivation of Lawsonia intracellularis, an obligate intracellular bacterium and the causative agent of proliferative enteropathy, was developed using an Original Space Bag inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% nitrogen. The flexibility of this protocol allows the testing of various environmental conditions for static cultivation of this bacterium and the development of diagnostic techniques.

