Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[In situ hybridization: principles, methods and applications].

J M Vignaud1, F Plenat, A Duprez

  • 1Laboratoire d'Anatomie Pathologique, Faculté de Médecine, Vandceuvre-Lès-Nancy, France.

Archives D'Anatomie Et De Cytologie Pathologiques
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative and comparative genomic analyses identify clinically relevant pulmonary carcinoid groups and unveil the supra-carcinoids.

Nature communications·2019
Same author

New Insights on Diagnostic Reproducibility of Biphasic Mesotheliomas: A Multi-Institutional Evaluation by the International Mesothelioma Panel From the MESOPATH Reference Center.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer·2018
Same author

Molluscum contagiosum surrounded by a white halo and Sezary syndrome.

Journal of the European Academy of Dermatology and Venereology : JEADV·2014
Same author

[The French mesothelioma network from 1998 to 2013].

Annales de pathologie·2014
Same author

Diagnostic value of immunohistochemistry for the detection of the BRAFV600E mutation in primary lung adenocarcinoma Caucasian patients.

Annals of oncology : official journal of the European Society for Medical Oncology·2012
Same author

[A rare rumor: pulmonary blastoma with pleural involvement].

Journal de radiologie·2011
Same journal

[Peritoneal pseudomyxoma associated with an ovarian and appendicular mucinous tumor].

Archives d'anatomie et de cytologie pathologiques·1999
Same journal

[Papillary fibroelastoma: a cardiac tumor rarely reported in children].

Archives d'anatomie et de cytologie pathologiques·1999
Same journal

[Extramembranous glomerulonephritis: clinicopathologic finding in a group of 45 Cameroonians].

Archives d'anatomie et de cytologie pathologiques·1999
Same journal

[Biotin-rich pseudoherpetic intranuclear inclusions. A diagnostic pitfall].

Archives d'anatomie et de cytologie pathologiques·1999
Same journal

[Primary gastric MALT lymphoma in children. Report of 2 cases].

Archives d'anatomie et de cytologie pathologiques·1999
Same journal

[Granular cell tumor and congenital epulis. Histochemical and immunohistochemical of 58 cases].

Archives d'anatomie et de cytologie pathologiques·1999
See all related articles

In situ hybridization visualizes DNA or RNA within cells and tissues. This technique involves preparing and labeling probes to identify specific genetic sequences in various biological samples.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Context:

  • In situ hybridization (ISH) is a powerful molecular technique.
  • It enables the direct visualization of nucleic acid targets within their native biological context.
  • Essential for understanding gene expression and localization in cells and tissues.

Purpose:

  • To describe the methodology of in situ hybridization.
  • To detail the preparation and labeling of nucleic acid probes.
  • To discuss the diverse applications of ISH in biological research.

Summary:

  • In situ hybridization (ISH) allows for the precise localization of specific DNA or RNA sequences.
  • The process involves using labeled probes that hybridize to complementary target sequences.

Related Experiment Videos

  • Key steps include probe design, labeling, hybridization, and detection within tissue sections, single cells, or chromosomes.

    • Impact:

    • Facilitates the study of gene expression patterns.
    • Aids in diagnosing genetic disorders and infectious diseases.
    • Provides critical insights into cellular function and tissue organization.