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Updated: May 26, 2026

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
Published on: July 4, 2016
Making a beta-barrel: assembly of outer membrane proteins in Gram-negative bacteria
Nathan W Rigel1, Thomas J Silhavy
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States.
None:
The outer membrane (OM) of Gram-negative bacteria is an essential organelle that serves as a selective permeability barrier by keeping toxic compounds out of the cell while allowing vital nutrients in. How the OM and its constituent lipid and protein components are assembled remains an area of active research. In this review, we describe our current understanding of how outer membrane proteins (OMPs) are delivered to and then assembled in the OM of the model Gram-negative organism Escherichia coli.
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