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Updated: May 26, 2026

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Published on: March 26, 2015

Cardiomyocyte apoptosis in heart development: methods and protocols.

Dongfei Qi¹, Mingui Fu

¹Department of Basic Medical Science and Shock/Trauma Research Center, School of Medicine, University of Missouri Kansas City, Kansas City, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 7, 2012

Summary

This summary is machine-generated.

Programmed cell death (apoptosis) is crucial in heart development. Researchers used advanced techniques to detect apoptosis in heart cells, aiding understanding of normal and abnormal cardiac development.

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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

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Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis

Published on: October 7, 2020

Area of Science:

Cardiology
Cell Biology
Developmental Biology

Background:

Programmed cell death, or apoptosis, plays a role in heart development.
Apoptosis is regulated by survival and death signals common to many tissues.
Understanding cardiomyocyte apoptosis is key to studying normal and abnormal heart development.

Purpose of the Study:

To investigate cardiomyocyte apoptosis during normal and abnormal heart development.
To apply and evaluate various cell death detection techniques in cardiac research.

Main Methods:

Utilized morphological, histological, and molecular assays for apoptosis detection.
Employed terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) for fragmented nuclei.
Applied cardiovascular molecular imaging with Annexin V and immunohistochemical detection of activated caspases.

Main Results:

The study details the application of multiple advanced techniques for detecting apoptosis in cardiac tissues.
These methods provide insights into the molecular mechanisms of cell death in the heart.
The findings contribute to the understanding of apoptosis's role in cardiac development.

Conclusions:

Advanced cell death detection techniques are essential for studying cardiomyocyte apoptosis.
These methods facilitate the investigation of apoptosis in both normal and pathological cardiac development.
Further research using these techniques can elucidate the precise role of apoptosis in heart formation and disease.