Related Experiment Videos
Endogenous digitalis-like factor as a stimulator of endothelin secretion from endothelial cells
1Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochemical and Biophysical Research Communications
|October 15, 1990
Abstract:
Effects of human urine-derived endogenous digitalis-like factor (EDLF) and ouabain on endothelin (ET) secretion were examined in cultured endothelial cells. ET was secreted in a linear fashion over 5 hours from bovine pulmonary artery endothelium into serum-free medium. EDLF stimulated ET secretion in a dose-dependent manner. In contrast, ouabain did not affect ET secretion at the concentration of 10(-9)-10(-5) M. These results indicate that human urine-derived EDLF is distinct from plant-derived ouabain and act as a stimulator of ET secretion by endothelial cells.