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Related Concept Videos

Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Gas Chromatography: Overview of Detectors01:13

Gas Chromatography: Overview of Detectors

Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: May 26, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

A highly sensitive fluorogenic chemodosimeter for rapid visual detection of phosgene.

Xuanjun Wu1, Zhisheng Wu, Yuhui Yang

  • 1Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province Xiamen University, Xiamen, China 361005.

Chemical Communications (Cambridge, England)
|January 10, 2012
PubMed
Summary

A new rhodamine-based chemodosimeter enables rapid, visual detection of phosgene, a toxic gas. This sensitive method achieves a detection limit of 50 nM for triphosgene, offering a practical safety tool.

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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

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Last Updated: May 26, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

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Published on: October 1, 2016

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Materials Science

Background:

  • Phosgene is a highly toxic industrial gas requiring sensitive detection methods.
  • Existing detection techniques may lack speed, visual output, or sensitivity.

Purpose of the Study:

  • To identify a highly sensitive chemodosimeter for phosgene detection.
  • To develop a rapid and visual method for phosgene sensing.

Main Methods:

  • Screening a panel of rhodamine derivatives.
  • Evaluating chemodosimeter sensitivity and visual response.
  • Testing detection of gaseous phosgene using treated paper strips.

Main Results:

  • A specific rhodamine derivative was identified as a highly sensitive chemodosimeter.
  • The chemodosimeter demonstrated a low detection limit of 50 nM for triphosgene.
  • Visual detection of gaseous phosgene was successfully achieved using paper strips.

Conclusions:

  • A novel rhodamine-based chemodosimeter provides a sensitive and visual method for phosgene detection.
  • The paper strip format offers a practical approach for rapid, on-site phosgene monitoring.