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Updated: May 26, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Fully enclosed microfluidic paper-based analytical devices.

Kevin M Schilling1, Anna L Lepore, Jason A Kurian

  • 1Department of Chemistry and Biochemistry, California Polytechnic State University, San Luis Obispo, California 93407, USA.

Analytical Chemistry
|January 11, 2012
PubMed
Summary
This summary is machine-generated.

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Fully enclosed microfluidic paper-based analytical devices (microPADs) were created using laser printing. This design enhances protection, reagent containment, and ease of use for low-cost diagnostics.

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Microfluidic paper-based analytical devices (microPADs) offer potential for low-cost diagnostics.
  • Existing microPAD designs can be susceptible to contamination and fluid evaporation.
  • Reagent storage and device handling present challenges for widespread microPAD adoption.

Purpose of the Study:

  • To develop a novel fabrication method for fully enclosed microPADs.
  • To evaluate the benefits of enclosed microfluidic channels for device performance and usability.
  • To enhance the suitability of microPADs for point-of-care diagnostic applications.

Main Methods:

  • Fabrication of microPADs using toner printing on both sides of paper via a laser printer.

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Microfluidic Applications for Disposable Diagnostics
10:21

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

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Last Updated: May 26, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics
03:58

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

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Microfluidic Applications for Disposable Diagnostics
10:21

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

  • Development of fully enclosed microfluidic channels.
  • Assessment of protection against contamination, reagent containment, fluid handling, and evaporation reduction.

    • Main Results:

    • Successfully fabricated fully enclosed microPADs using a simple laser printing technique.
    • Demonstrated enhanced protection of microfluidic channels from external contamination.
    • Confirmed improved containment of reagents and fluids, leading to easier handling.
    • Observed a significant reduction in solution evaporation from the channels.

    Conclusions:

    • Fully enclosed microPADs fabricated by toner printing offer significant advantages over conventional designs.
    • The enhanced protection and usability make these devices more robust for real-world applications.
    • This fabrication method advances the development of low-cost, reliable point-of-care diagnostic tools.