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Updated: May 26, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
Published on: September 3, 2013
Spatial manipulation of cells and organelles using single electrode dielectrophoresis
David M Graham1, Mark A Messerli, Ronald Pethig
1The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Woods Hole, MA, USA. dgraham@mbl.edu
Abstract:
The selection, isolation, and accurate positioning of single cells in three dimensions are increasingly desirable in many areas of cell biology and tissue engineering. We describe the application of a simple and low cost dielectrophoretic device for picking out and relocating single target cells. The device consists of a single metal electrode and an AC signal generator. It does not require microfabrication technologies or sophisticated electronics. The dielectrophoretic manipulator also discriminates between live and dead cells and is capable of redistributing intracellular organelles.
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