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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
Mapping of vascular ZIP codes by phage display.
Tambet Teesalu1, Kazuki N Sugahara, Erkki Ruoslahti
1Center for Nanomedicine, Sanford-Burnham Medical Research Institute at UCSB, Santa Barbara, California, USA.
Methods in Enzymology
|January 11, 2012
Summary
Vascular targeting can be achieved using unique peptide ligands identified through in vivo phage display. Certain peptides, like CendR, facilitate payload delivery into tissues, offering new therapeutic strategies.
Area of Science:
- Biotechnology
- Molecular Biology
- Pharmacology
Background:
- Organs and pathologies possess unique vascular signatures ('ZIP codes') targetable by affinity ligands.
- In vivo peptide phage display enables unbiased mapping of vascular diversity.
- Identified peptides can bind vessels and elicit biological responses, including tissue penetration.
Purpose of the Study:
- To provide a practical guide for establishing ex vivo and in vivo phage display.
- To highlight the utility of phage display for vascular targeting and payload delivery.
- To introduce the T7 coliphage platform for its versatility and ease of use.
Main Methods:
- In vivo peptide phage display for vascular mapping.
- Identification and characterization of affinity ligands and tissue-penetrating peptides.
- Focus on the T7 coliphage system for phage display applications.
Main Results:
- Phage display successfully maps unique vascular targets.
- Certain peptides demonstrate both binding and biological activity.
- CendR peptides enable payload extravasation and parenchymal spread.
Conclusions:
- Phage display is a powerful tool for discovering vascular-targeting ligands.
- CendR peptides offer a novel mechanism for drug delivery into tissues.
- The T7 coliphage platform is a versatile choice for phage display research.
