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Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome

Mitsuhiro Itaya1

  • 1Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, Japan. mita2001@sfc.keio.ac.jp

Bioscience, Biotechnology, and Biochemistry
|January 11, 2012
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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