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[The effect of total phenolics from abnormal savda munziq on cervical cancer cell lines]
Ke-Yao Chen1, Ablimit Tangnur, Guo-Ning Wang
1Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China. 656262393@qq.com
Objective:
To explore the feasibility of total phenolics from Abnormal Savda Munziq on treating human cervical carcinoma.
Methods:
MTT assay was used to determine the growth inhibition ratio and IC50 on Hela cells and SiHa cells after treated with different concentrations of total phenolics. Morphologic changes of cells were observed by inverted microscope.
Results:
Total phenolics inhibited the proliferation of Hela cells in the concentration range of 25 - 125 microg/mL; Total phenolics inhibited the proliferation of SiHa cells in the concentration range of 75 - 175 microg/mL; The inhibitory actions of total phenolics on the Hela and SiHa appeard dose-effect relationship and time-effect relationship obviously (P < 0.01); The IC50 of Hela was (125.26 +/- 16.15) microg/mL after 48h total phenolics treatment; The IC50 of SiHa was (134.51 +/- 2.55) microg/mL after 48h total phenolics treatment. And there was no statistical sense in the disparation of them (P > 0.05); Both of the cells showed apoptosis character evidently after total phenolics treatment, Along with the increasing of the concentration and the action time, morphologic changes of cells were more obviously.
Conclusion:
Total phenolics could inhibit the growth of Hela cells and SiHa cells and the inhibitory actions of total phenolics on the two cells is almostly the same. Therefore, total phenolics from abnormal savda munziq is deserved to be further studied for treating human cervical carcinoma.
Insights
Total phenolics from Abnormal Savda Munziq show potential in inhibiting human cervical carcinoma cells. Further research is warranted to explore its therapeutic feasibility for cervical cancer treatment.
Area of Science:
- Phytochemistry
- Oncology
- Cell Biology
Context:
- Human cervical carcinoma is a significant global health concern.
- Exploring novel therapeutic agents from natural sources is crucial for cancer treatment.
Purpose:
- To investigate the anti-cancer effects of total phenolics extracted from Abnormal Savda Munziq on human cervical carcinoma cell lines (Hela and SiHa).
Summary:
- Total phenolics demonstrated significant inhibition of Hela and SiHa cell proliferation in a dose- and time-dependent manner.
- The half-maximal inhibitory concentration (IC50) values for Hela and SiHa cells were comparable.
- Morphological analysis confirmed apoptosis induction in cancer cells upon treatment, suggesting a cytotoxic mechanism.
Impact:
- These findings suggest that total phenolics from Abnormal Savda Munziq possess anti-proliferative and apoptotic properties against cervical cancer cells.
- This study supports the potential of Abnormal Savda Munziq as a source for developing novel botanical-based therapies for cervical carcinoma.
