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[Process optimization for extraction of immune active polysaccharides from Fomes fomentarius by introduction of
Gai-Yi-Nan He1, Lin-Sheng Lei, Jie Chen
1School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. hegaishan@sina.com.cn
Objective:
To study the optimal extraction process of immune active polysaccharides from Fomes fomentarius by introduction of ultrasonication.
Methods:
An orthogonal experimental design of L9 (3(4)) was used to investigate the effects of ultrasonication time, extraction temperature and extraction time on the extraction ratio, sugar content and immune stimulating activity (mouse splenocyte metabolic activity measured with MTT colorimetry) of the polysaccharides and the optimal extraction process was evaluated.
Results:
Ultrasonication treatment had the most remarkable effect on the immune stimulating activity of the polysaccharides. The optimal extraction process for extraction of immune active polysaccharides was as follows: ultrasonication for 30min, extraction temperature at 80 degrees C and water extraction time for 2h.
Conclusion:
Ultrasonication can be used as a useful technique for extraction of immune active polysaccharides from Fomes fomentarius.
