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Updated: May 25, 2026

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae
Published on: November 17, 2023
"Point" centromeres of Saccharomyces harbor single centromere-specific nucleosomes
Steven Henikoff1, Jorja G Henikoff
1Basic Sciences Division, Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA. steveh@fhcrc.org
Abstract:
The "point" centromere of budding yeast is genetically defined by an ≈ 125-bp sequence. Recent fluorescence measurements of kinetochore clusters have suggested that this sequence specifies multiple centromere histone 3 (CenH3) nucleosomes. However, high-resolution mapping demonstrates that there is only one CenH3 nucleosome per centromere, providing biochemical confirmation of the point centromere model.
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