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Related Experiment Video

Updated: May 25, 2026

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

Published on: May 3, 2024

Short peptide modules for enhancing intestinal barrier function.

Soichi Tanabe¹

¹Hiroshima University, Graduate School of Biosphere Science, 1-4-4 Kagamiyama, Higashi-hiroshima, 739-8528, Japan. stanabe@hiroshima-u.ac.jp

Current Pharmaceutical Design

|January 13, 2012

Summary

A milk-derived peptide, Asn-Pro-Trp-Asp-Gln (NPWDQ), enhances intestinal barrier function by upregulating occludin. This discovery offers potential for preventing intestinal disorders like inflammatory bowel disease.

Related Experiment Videos

Last Updated: May 25, 2026

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

Published on: May 3, 2024

Area of Science:

Gastroenterology
Immunology
Molecular Biology

Background:

The intestinal epithelial barrier is crucial for immune function and its defects are linked to inflammatory bowel diseases, food allergies, and celiac disease.
Modulating intestinal barrier function is a promising pharmacological target for treating intestinal disorders.

Purpose of the Study:

To investigate the effect of a specific milk-derived peptide, Asn-Pro-Trp-Asp-Gln (NPWDQ), on intestinal barrier function.
To explore the molecular mechanisms by which NPWDQ influences the intestinal barrier.

Main Methods:

Hydrolysis of casein to obtain the peptide NPWDQ.
Inhibition of ovalbumin permeation in Caco-2 human intestinal cells.
DNA microarray analysis to assess gene expression changes.
Western blot analysis to confirm protein expression levels.

Main Results:

NPWDQ significantly inhibited ovalbumin permeation in Caco-2 cells, indicating enhanced barrier function.
NPWDQ specifically up-regulated the expression of the occludin gene.
Increased occludin protein levels were observed, confirming the molecular effect of NPWDQ.

Conclusions:

Milk-derived peptides, such as NPWDQ, can enhance intestinal barrier function.
NPWDQ's specific action on occludin suggests a targeted mechanism for improving barrier integrity.
These findings highlight the potential of milk-derived peptides for preventing and managing intestinal disorders.