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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
Published on: June 29, 2016
Reactivity of a human monoclonal antibody against Rh D with the intermediate filament protein vimentin
1Division of Haematology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire.
Abstract:
The human IgM anti-Rh D antibody MAD-2 has previously been shown to react with human and animal tissues and leucocytes. Double labelling immunofluorescence with MAD-2 and a mouse monoclonal antibody against vimentin, the intermediate filament protein of cells of mesenchymal origin, showed coincidental staining which was distinct from that seen with antibodies against other cytoskeletal proteins. Using immunoblotting, both MAD-2 and anti-vimentin reacted with a 55 kDa tissue component, and with purified vimentin. These results show that the major tissue and leucocyte protein recognized by MAD-2 is vimentin.
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