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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: May 25, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
13:32

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

DNA extraction from fossil eggshell.

Charlotte L Oskam1, Michael Bunce

  • 1Ancient DNA Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, South Street, Perth, 6150, WA, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|January 13, 2012
PubMed
Summary
This summary is machine-generated.

Ancient DNA (aDNA) can be recovered from fossil avian eggshells. This optimized protocol efficiently extracts aDNA from eggshells, minimizing inhibitors for reliable ancient DNA analysis.

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Area of Science:

  • Paleogenomics
  • Molecular Archaeology
  • Paleontology

Background:

  • Avian eggshell fragments from paleontological and archaeological sites preserve ancient DNA (aDNA).
  • Extraction of high-quality aDNA from fossil eggshells is challenging due to inhibitors co-purifying with DNA.

Purpose of the Study:

  • To describe an optimized DNA extraction protocol for avian eggshell fragments.
  • To maximize ancient DNA recovery while minimizing PCR inhibitors.

Main Methods:

  • Powdering of fossil eggshell fragments.
  • Digestion and heating to release DNA from the calcite matrix.
  • DNA concentration followed by purification and washing using silica columns.

Main Results:

  • Successful recovery of ancient DNA from diverse extinct avian species, including moa, elephant birds, and emu.
  • The protocol effectively isolates aDNA from eggshell matrices up to 19,000 years old.
  • Minimized co-purification of PCR inhibitors, enabling downstream applications.

Conclusions:

  • This optimized protocol provides a reliable method for ancient DNA extraction from avian eggshells.
  • The technique significantly enhances the potential for paleogenomic studies using fossil eggshell material.