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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: May 25, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

DNA extraction from formalin-fixed material.

Paula F Campos¹, Thomas M P Gilbert

¹Natural History Museum of Denmark, University of Copenhagen, Østervoldgade 5-7, 1350 Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|January 13, 2012

Summary

This summary is machine-generated.

This study introduces an efficient DNA extraction protocol for formalin-fixed paraffin-embedded (FFPE) tissues. The method reverses protein-DNA cross-links, yielding longer DNA fragments for improved PCR-based analyses.

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Last Updated: May 25, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

Area of Science:

Molecular Biology
Biochemistry
Genomics

Background:

Polymerase Chain Reaction (PCR) analysis of DNA from formalin-fixed materials faces challenges like DNA fragmentation and protein-DNA cross-links.
These issues limit the quality and quantity of amplifiable DNA, hindering downstream applications.

Purpose of the Study:

To develop an efficient protocol for extracting high-quality DNA from formalin-fixed paraffin-embedded (FFPE) tissues.
To overcome the limitations of DNA fragmentation and protein-DNA cross-linking in FFPE samples.

Main Methods:

A novel protocol involving heat and alkali treatment to reverse protein-DNA cross-links.
Extraction of DNA from formalin-fixed or paraffin-embedded tissues (FFPE).

Main Results:

The developed protocol significantly reverses protein-DNA cross-links.
Yields longer DNA fragments compared to standard extraction methods.
Increases the amount of PCR-amplifiable DNA from FFPE tissues.

Conclusions:

This efficient protocol enhances DNA quality and quantity from FFPE samples.
It provides a valuable tool for improving PCR-based analyses on archival tissues.
The method offers a significant improvement over existing DNA extraction techniques for FFPE specimens.