Related Experiment Video
Updated: May 25, 2026

Mapping Dysfunctional Protein-Protein Interactions in Disease
Published on: October 24, 2025
A new approach to high sensitivity liquid chromatography-mass spectrometry of peptides using nanoflow solvent
Beixi Wang1, Ellen D Inutan, Sarah Trimpin
1Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Abstract:
Liquid chromatography (LC) solvent assisted inlet ionization (SAII) mass spectrometry (MS) was previously reported to give good chromatographic resolution and MS detection injecting 66 ng of a BSA tryptic digest. In analogy to nano-electrospray ionization (nESI), we extend SAII LC/MS to nano-SAII (nSAII) operating at nL min(-1) flow rates and demonstrate good quality ion chromatograms and mass spectra from injection of as little as 0.7 ng of BSA digest onto a capillary LC column. Data dependent fragmentation is demonstrated for injection of 7 ng of a BSA digest. This method has advantages over nESI in ease of use and low cost as it requires no voltage and is operational without the necessity of connectors or fragile nESI emitters, although similar constricted tips can be helpful in nSAII to stabilize the signal at low nanoliter flow. At a flow rate of 0.8 μL min(-1), the only requirement for nSAII is that the exit-end of the capillary LC column be adjusted near the aperture of the heated inlet of the mass spectrometer.
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Types of Detectors

