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Updated: May 25, 2026

Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry
Published on: April 26, 2024
Methane production on thickened, pre-fermented manure.
Erik R Coats1, Ibrahim Ibrahim, Aurelio Briones
1Department of Civil Engineering, University of Idaho, P.O. Box 441022, Moscow, ID 83844-1022, USA. ecoats@uidaho.edu
A novel two-stage anaerobic digestion (AD) process effectively converts pre-fermented dairy manure into methane-rich biogas. This method enhances biogas methane content and maintains process stability, similar to using raw manure.
Area of Science:
- Environmental Science
- Biotechnology
- Agricultural Engineering
Background:
- Dairy cows in the US produce over 226 billion kg of manure annually.
- Manure management is a significant environmental challenge in the dairy industry.
- Anaerobic digestion (AD) is a promising technology for waste valorization and renewable energy production.
Purpose of the Study:
- To evaluate a novel two-stage anaerobic digestion (AD) process for dairy manure.
- To assess the biogas yield and quality from pre-fermented dairy manure using this new method.
- To compare the performance and stability of the two-stage AD system with conventional AD using raw manure.
Main Methods:
- Utilized a two-stage anaerobic digestion (AD) system.
- Fed the system with thickened, pre-fermented dairy manure.
- Analyzed biogas quantity, methane content, stoichiometry, and microbial communities, focusing on Methanosarcinaceae (Msc).
Main Results:
- The two-stage AD of pre-fermented manure produced biogas yields comparable to raw manure AD.
- Biogas from the pre-fermented manure showed enhanced methane content.
- The two-stage system demonstrated comparable process stability and biogas stoichiometry to raw manure AD.
- The two-stage AD process resulted in a higher abundance of acetoclastic methanogens (Methanosarcinaceae).
Conclusions:
- Two-stage anaerobic digestion is a viable method for producing high-quality biogas from pre-fermented dairy manure.
- Enhanced enrichment of Methanosarcinaceae in the two-stage system contributes to stable and efficient biogas production.
- This process offers a sustainable solution for dairy manure management and renewable energy generation.
Related Concept Videos
Microbes and Methanogenesis
Production of Organic Acids
Microbes in the Production of Fermented Foods
Microbes in Food Production
Microbial Interactions: Mutualism
Microbes and Climate Change

