Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbes and Methanogenesis

Microbes and Methanogenesis

Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

Microbes in the Production of Fermented Foods

Microbes in the Production of Fermented Foods

Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...

Microbes in Food Production

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...

Microbial Interactions: Mutualism

Microbial Interactions: Mutualism

Mutualism is a symbiotic interaction in which all participating organisms benefit. These relationships can be obligate or facultative and are fundamental to ecosystem functions across diverse biological systems.Plant–Fungi MutualismOne well-known example is the association between plant roots and mycorrhizal fungi, such as Rhizophagus species. The fungal hyphae penetrate the root hairs and the epidermis, forming an extensive hyphal network that establishes a symbiotic association. Through this...

Microbes and Climate Change

Microbes and Climate Change

Microorganisms are pivotal agents in Earth's biogeochemical cycles, significantly influencing climate dynamics through their metabolic activities. These microbes modulate the levels of key greenhouse gases by both contributing to and helping mitigate climate change.Microbial Contributions to Greenhouse Gas EmissionsRising global temperatures accelerate microbial metabolism, which, in turn, speeds up the decomposition of organic matter. This process releases carbon dioxide (CO₂) through...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

VITA Accelerator Neutron Sources: Status and Research Results.

Cancers·2026

Same author

A call to action for the use of composite hematologic measurements in hereditary hemorrhagic telangiectasia and other chronic bleeding disorders.

Expert review of hematology·2026

Same author

Internally stored carbon-driven full and partial denitrification: mechanisms and implications for wastewater treatment applications.

Water research·2026

Same author

Bridging Microbiomes: Exploring Oral and Gut Microbiomes in Autoimmune Thyroid Diseases- New Insights and Therapeutic Frontiers.

Gut microbes reports·2026

Same author

Diabetes in Pregnancy: A Review of Service Provision and Practice at a Maternity Center.

Life (Basel, Switzerland)·2026

Same author

Management of Hypothyroidism in Pregnancy and Its Impact on Maternal and Perinatal Outcomes: A Single-Center Retrospective Cohort Study.

Life (Basel, Switzerland)·2026

Search research articles

Related Experiment Video

Updated: May 25, 2026

Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry

Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry

Published on: April 26, 2024

Methane production on thickened, pre-fermented manure.

Erik R Coats¹, Ibrahim Ibrahim, Aurelio Briones

¹Department of Civil Engineering, University of Idaho, P.O. Box 441022, Moscow, ID 83844-1022, USA. ecoats@uidaho.edu

Bioresource Technology

|January 14, 2012

Summary

A novel two-stage anaerobic digestion (AD) process effectively converts pre-fermented dairy manure into methane-rich biogas. This method enhances biogas methane content and maintains process stability, similar to using raw manure.

More Related Videos

The Use of an Automated System (GreenFeed) to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals

The Use of an Automated System (GreenFeed) to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals

Published on: September 7, 2015

Lab-Scale Model to Evaluate Odor and Gas Concentrations Emitted by Deep Bedded Pack Manure

Lab-Scale Model to Evaluate Odor and Gas Concentrations Emitted by Deep Bedded Pack Manure

Published on: July 19, 2018

Related Experiment Videos

Last Updated: May 25, 2026

Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry

Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry

Published on: April 26, 2024

The Use of an Automated System (GreenFeed) to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals

The Use of an Automated System (GreenFeed) to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals

Published on: September 7, 2015

Lab-Scale Model to Evaluate Odor and Gas Concentrations Emitted by Deep Bedded Pack Manure

Lab-Scale Model to Evaluate Odor and Gas Concentrations Emitted by Deep Bedded Pack Manure

Published on: July 19, 2018

Area of Science:

Environmental Science
Biotechnology
Agricultural Engineering

Background:

Dairy cows in the US produce over 226 billion kg of manure annually.
Manure management is a significant environmental challenge in the dairy industry.
Anaerobic digestion (AD) is a promising technology for waste valorization and renewable energy production.

Purpose of the Study:

To evaluate a novel two-stage anaerobic digestion (AD) process for dairy manure.
To assess the biogas yield and quality from pre-fermented dairy manure using this new method.
To compare the performance and stability of the two-stage AD system with conventional AD using raw manure.

Main Methods:

Utilized a two-stage anaerobic digestion (AD) system.
Fed the system with thickened, pre-fermented dairy manure.
Analyzed biogas quantity, methane content, stoichiometry, and microbial communities, focusing on Methanosarcinaceae (Msc).

Main Results:

The two-stage AD of pre-fermented manure produced biogas yields comparable to raw manure AD.
Biogas from the pre-fermented manure showed enhanced methane content.
The two-stage system demonstrated comparable process stability and biogas stoichiometry to raw manure AD.
The two-stage AD process resulted in a higher abundance of acetoclastic methanogens (Methanosarcinaceae).

Conclusions:

Two-stage anaerobic digestion is a viable method for producing high-quality biogas from pre-fermented dairy manure.
Enhanced enrichment of Methanosarcinaceae in the two-stage system contributes to stable and efficient biogas production.
This process offers a sustainable solution for dairy manure management and renewable energy generation.