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Published on: June 26, 2018
[Screen for natural benzylisoquinoline alkaloids against tumor]
Zhengfu Zhang1, Ying Guo, Xionghui Wei
1Department of Applied Chemistry, College of Chemistry & Molecular Engineering, Peking University, Beijing 100871, China. zzfbj@sina.com
Objective:
To search for natural benzylisoquinoline alkaloids against tumor.
Method:
In this study, taken DNA as target, a method was developed for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine by the use of centrifugal ultrafiltration combined with HPLC technology. The anti-tumor activity for the compounds screened was evaluated in vitro.
Result:
Nine compounds interacted with DNA were discovered and identified from Macleaya cordata, Chelidonium majus, Coptis chinensis, and the proliferation of four types of human solid cancer cell lines was markedly inhibited by these compounds.
Conclusion:
The developed method was considered to be suitable for screening of natural benzylisoquinoline alkaloids against tumor from traditional Chinese medicine.
