Related Experiment Video
Updated: May 25, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Published on: December 18, 2019
Ameloblastin upstream region contains structural elements regulating transcriptional activity in a stromal cell line
Margareth V Tamburstuen1, Malcolm L Snead, Janne E Reseland
1Department of Biomaterials, Institute for Clinical Dentistry, University of Oslo (UiO), Oslo, Norway.
Abstract:
Ameloblastin (AMBN) was originally described as a tooth-specific extracellular matrix protein, but current data have shown that AMBN is present in many different tissues of mesenchymal origin. The identification of regulatory elements in the promoter region of the Ambn gene would assist in identifying potential mesenchymal-specific transcriptional factors. In this study we subcloned a 3,788-bp region upstream (and a 54-bp region downstream) of the mouse Ambn transcriptional start site into a LacZ reporter construct and called this construct 3788-Ambn-lacZ. In silico analysis of the 3,788-bp Ambn promoter region identified 50 potential cis-regulatory elements, 29 of which are known to be functional in cell populations of mesenchymal origin. The reporter construct was activated in transfected bone marrow cells, and the promoter activity was induced in cell cultures following addition of recombinant AMBN, interferon-γ, serotonin, or dexamethasone. We discuss the relative significance of the potential cis-acting gene-regulatory elements of Ambn in relation to bone morphogenesis. Knowledge of Ambn gene-regulatory elements will be of importance when developing strategies for bone repair and replacement in a clinical surgical setting.
Related Concept Videos
Master Transcription Regulators
General Transcription Factors
RNA Polymerase II Accessory Proteins
The Eukaryotic Promoter Region
Cell Specific Gene Expression
TGF - β Signaling Pathway

