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Updated: May 25, 2026

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Published on: April 28, 2022
The methylproteome and the intracellular methylation network
Melissa A Erce1, Chi N I Pang, Gene Hart-Smith
1Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Protein methylation, a post-translational modification (PTM), is crucial for cellular processes beyond epigenetics, significantly regulating protein interactions and influencing systems biology. This review explores methylation's role in the dynamic interactome.
Area of Science:
- Biochemistry
- Molecular Biology
- Systems Biology
Background:
- Post-translational modification (PTM) via protein methylation is a critical cellular process.
- While well-known for its role in the epigenetic code, methylation's influence extends to broader cellular functions.
- Emerging evidence highlights methylation as a key regulator of protein-protein interactions and interactome dynamics.
Purpose of the Study:
- To review the role of protein methylation in regulating protein-protein interactions.
- To illustrate the impact of methylation on systems biology and interactome dynamics.
- To discuss analytical techniques for studying the methylproteome and methylation's role in pathology.
Main Methods:
- Literature review focusing on protein methylation and interactome dynamics.
- Summary of current knowledge on methylation's impact on systems biology.
- Discussion of analytical techniques for methylproteome analysis.
Main Results:
- Methylation plays a significant role in regulating protein-protein interactions, affecting interactome dynamics.
- Methylation can facilitate or inhibit protein-protein interactions, often in interplay with other PTMs like phosphorylation.
- Methylation is implicated in pathology-associated protein interaction networks, with examples including human diseases and the p53 protein.
Conclusions:
- Protein methylation is a fundamental regulator of interactome dynamics with broad implications in systems biology.
- Understanding methylproteome dynamics is crucial for deciphering cellular processes and disease mechanisms.
- Advanced analytical techniques are essential for elucidating the complex roles of methylation in protein interactions.
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