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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Updated: May 25, 2026

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lensless fluorescent on-chip microscopy using a fiber-optic taper.

Ahmet F Coskun1, Ikbal Sencan, Ting-Wei Su

  • 1Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|January 19, 2012
PubMed
Summary

This study presents a lensfree on-chip fluorescent microscopy platform for wide-field imaging of fluorescently labeled cells. The innovative system achieves high resolution and could advance applications in cytometry and rare cell detection.

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Last Updated: May 25, 2026

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Area of Science:

  • Biomedical Engineering
  • Optical Microscopy
  • Nanotechnology

Background:

  • Traditional microscopy faces limitations in field-of-view and portability.
  • Lensfree imaging offers a compact alternative for biological sample analysis.
  • Fiber-optic faceplates enable enhanced light collection and resolution in micro-imaging systems.

Purpose of the Study:

  • To develop and validate a lensfree on-chip fluorescent microscopy platform.
  • To achieve wide-field imaging with high spatial resolution for biological samples.
  • To explore applications in cytometry, rare cell imaging, and microarray research.

Main Methods:

  • Utilizing a tapered fiber-optic faceplate for direct sample imaging.
  • Employing a simple incoherent light source (LED) for fluorescence excitation.
  • Implementing a compressive sampling algorithm for signal recovery and enhanced space-bandwidth-product.

Main Results:

  • Demonstrated imaging of fluorescently labeled cells over a ~60 mm(2) field-of-view.
  • Achieved <4 µm spatial resolution.
  • Validated performance with fluorescent micro-particles and labeled water-borne parasites (Giardia Muris cysts).

Conclusions:

  • The developed lensfree platform offers a compact and wide-field solution for fluorescent imaging.
  • The system shows promise for high-throughput cytometry and sensitive detection of rare cells.
  • This technology could significantly impact micro-array research and point-of-care diagnostics.