A computer program in BASIC for calculating gradients used in chromatography, electrophoresis and centrifugation

Area of Science:

• Analytical Chemistry
• Biochemistry
• Physical Chemistry

Background:

• Concentration gradients are fundamental in various separation sciences.
• Techniques like chromatography, electrophoresis, and centrifugation rely on precise gradient formation.
• Accurate calculation and graphical representation of gradients are crucial for optimizing separation efficiency.

Purpose of the Study:

• To describe a BASIC computer program for calculating and graphically representing concentration gradients.
• To provide a tool for researchers utilizing gradient-based separation methods.
• To enhance the accessibility and usability of gradient calculations for scientific applications.

Main Methods:

• Development of a BASIC computer program named GRADIENT.
• Implementation of algorithms for calculating concentration gradient profiles.
• Generation of graphical representations of the calculated gradients.
• Program designed for IBM-compatible personal computers.

Main Results:

• A functional computer program (GRADIENT) capable of calculating and plotting concentration gradients.
• The program simplifies the complex task of gradient design for separation experiments.
• Graphical output aids in understanding and predicting separation behavior.

Conclusions:

• The GRADIENT program offers a practical and accessible solution for researchers involved in separation science.
• Computer-aided gradient calculation and visualization can improve the reproducibility and success rate of chromatographic, electrophoretic, and centrifugal separations.
• This tool can facilitate experimental design and troubleshooting in gradient-based analytical techniques.