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[Study on HPLC fingerprint of Alpinia officinarum]
Yi-Feng Deng1, Li-Na Feng, Hui Luo
1Guangdong Medical College, Key laboratory of Natural Drugs Research and Development of Guangdong, Zhanjiang 524023, China. dengyfgd@163.com
Objective:
To establish the chromatography fingerprint of Alpinia officinarum by HPLC.
Methods:
An optimum HPLC conditions which were obtained under the assessment of LC-MS were as follows: Shim-pack VP-ODS column (2.0 mm x 250 mm, 5 microm), 0.1% HAc aqueous solution as phase A, 15% Acetonitrile: 40% Methanol: 45% Tetrafuran as phase B, the flow rate was 0.20 mL/min, column temperature was 35 degrees C and UV detector was set at 280 nm.
Results:
The HPLC fingerprint of Alpinia officinarum was established, the consensus 10 peaks and their relative retention times along with the ranges of relative area were determined.
Conclusion:
The method is reliable and stable and can be used for the quality control and identification of Alpinia officinarum.
