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[Identification of marsdeniae tenacissimae caulis and its adulterants by RAPD]
Hui Zhang1, Zhi-Dong Pei, Chong Ni
1Liaoning Universitiy of Chinese Traditional Medicine, Dalian 116600, China. syyycs@163.com
Objective:
To identify Marsdeniae Tenacissimae Caulis and its adulterants by RAPD (random amplified polymorphic DNA) and analyze the gene homology of Marsdeniae Tenacissimae Caulis from various habitats.
Methods:
General DNA was isolated from Marsdeniae tenacissimae Caulis which were from seven various habitats and its six adulterants by CTAB, the twenty RAPD was used to identify them.
Results:
Random primer 285 (GGG AAC CCT T) could amplify the gene of Marsdeniae tenacissimae Caulis from various habitats stablely, Marsdeniae tenacissimae Caulis and its adulterants could be identified by primer E01 (CCC AAG GTC C) effectively.
Conclusion:
The method of RAPD can be used to identify Marsdeniae Tenacissimae Caulis and its adulterants, the gene of Marsdeniae tenacissimae Caulis from various habitats have homology.
