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Updated: May 25, 2026
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
[Preparation conditions optimization of Epimedium polysaccharide liposome]
Huan Gao1, Qi Gao, De-Yun Wang
1Institute of Traditional Chinese Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. 2009107131@njau.edu.cn
Objective:
To optimize the preparation conditions of Epimedium polysaccharide liposome (EPSL).
Methods:
EPSL was prepared by membrane distribution-supersound method. In preparation of EPSL test, an orthogonal L9 (3(4)) test was used to optimize the preparation conditions, with four factors of ratio of drug to lipid, ratio of lecithin to cholesterol, ultrasonic time and water bath temperature and two indexes of encapsulation efficiency and drug-loading rate. The encapsulation efficiency of EPSL was determined by protamine method.
Results:
Optimal preparation condition of EPSL was that ratio of drug to lipid, ratio of lecithin to cholesterol, ultrasonic time and water bath temperature were 1: 30, 4: 1,10 min and 40 degrees C respectively.
Conclusion:
EPSL prepared under Optimal conditions has the property of high encapsulation efficiency and drug-loading rate.
