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Updated: May 25, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
[Advance in detection methods of microbes on historic stones--a review]
Miao Yu1, Xudong Zhu, Jiao Pan
1National Key Subject of Microbiology, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China. shuilifang@mail.nanki.edu.cn
Abstract:
We reviewed the methods for identification of microorganisms on the surface of historic stones, including nucleic acid analysis, cell membrane analysis, secondary metabolites analysis and the traditional culture analysis. After comprehensive comparison of the advantages and disadvantages of each method, we addressed the biological protection of stone artifacts. The establishment of rapid detection of microorganisms on the historic stones is important to prevent corrosion caused by microorganisms and to protect our valuable cultural heritage.
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