Related Experiment Video
Updated: May 25, 2026

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Published on: October 26, 2016
Fast characterization of polyelectrolyte complexes by inline coupling of capillary electrophoresis to Taylor
Laurent Leclercq1, Hervé Cottet
1Institut des Biomolécules Max Mousseron, UMR CNRS 5247, Université de Montpellier 1, Université de Montpellier 2, 15 Avenue Charles Flahault 34060 Montpellier, France.
Abstract:
The inline coupling of capillary electrophoresis (CE) to Taylor dispersion analysis was used for the characterization of polyelectrolyte complexes. The charge stoichiometry and the hydrodynamic radii of the two polyelectrolyte constituents were determined in a fully automated single run using standard commercial CE apparatus with a single detection point. The proposed methodology utilizes unusual high ionic strength (1.3 M) background electrolyte to obtain the dissociation and electrophoretic separation of polyelectrolyte constituents. Such highly saline conditions in combination with neutrally coated capillary were found to avoid any polyelectrolyte interactions onto the capillary surface. This innovative methodology should greatly contribute to simplify and accelerate the characterization of polyelectrolyte complexes or polyplexes.
Related Concept Videos
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary Electrophoresis: Instrumentation
Ion-Exchange Chromatography
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

