[Determination of three polycyclic aromatic hydrocarbons in mainstream cigarette smoke by automated solid phase...

A simple method for the analysis of benzo [ a ] pyrene, benzo [ a ] anthracene and chrysene in mainstream cigarette smoke using automated solid phase extraction (SPE) system-gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. After being smoked in the ISO mode, the Cambridge filter pad was used to collect mainstream cigarette smoke, and then extracted by 40 mL cyclohexane containing deuterated benzo [a] pyrene as the internal standard. The extract was cleaned-up by an automated PrepLinc SPE-AccuVap system and then detected using GC-MS/MS. The detection limits for benzo [a] pyrene, benzo [a] anthracene and chrysene were 0.05,0. 16, and 0.23 ng/cig, respectively. The recoveries were ranged from 91.5% to 102.1% to 102.1% with the relative standard deviations (RSDs) less than 5%. This method was proved to be simple, sensitive, and suitable for the determination of benzo [a] pyrene, benzo[a] anthracene and chrysene in mainstream cigarette smoke.