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Quality-Controlled Sputum Analysis by Flow Cytometry
Published on: August 9, 2021
Flow cytometry: retrospective, fundamentals and recent instrumentation
Julien Picot1, Coralie L Guerin, Caroline Le Van Kim
1Institut National de la Transfusion Sanguine, 75739, Paris Cedex 15, France, jpicot@ints.fr.
Cytotechnology
|January 25, 2012
Summary
Flow cytometry is a powerful cell analysis technology. This review covers its fundamentals and recent advancements in instrumentation for precise cellular studies.
Area of Science:
- Biotechnology
- Cell Biology
- Analytical Chemistry
Background:
- Flow cytometry is a key technology for high-precision cellular population analysis.
- It integrates sample handling, rapid data acquisition, and multiple measurements.
- The technique enables physical separation of specific cell subpopulations.
Purpose of the Study:
- To provide a comprehensive historical overview of flow cytometry.
- To review the fundamental principles of flow cytometry.
- To discuss recent developments in commercial flow cytometry instrumentation.
Main Methods:
- Literature review and historical analysis.
- Review of fundamental principles of fluidics, optics, and electronics in flow cytometry.
- Analysis of current commercial instrumentation features and capabilities.
Main Results:
- Flow cytometry has evolved significantly due to interdisciplinary collaboration.
- The technology offers high precision, speed, and multiplexing capabilities.
- Recent instrumentation shows advancements in sensitivity, throughput, and data analysis.
Conclusions:
- Flow cytometry is a mature and versatile technology in biological research.
- Understanding its fundamentals is crucial for appreciating its capabilities.
- Continuous innovation in instrumentation enhances its application scope.
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