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Chiral separation of vinpocetine using cyclodextrin-modified micellar electrokinetic chromatography
Wan Aini Wan Ibrahim1, Siti Munirah Abd Wahib, Dadan Hermawan
1Separation Science and Technology Group, Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, Malaysia. wanaini@kimia.fs.utm.my
Abstract:
A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique has been developed for enantioseparation of vinpocetine using an inexpensive 2-hydroxypropyl-β-CD (HP-β-CD) as the chiral selector (CS). The best chiral separation was achieved using 40 mM HP-β-CD as the CS in 50 mM phosphate buffer (pH 7.0) consisting of 40 mM sodium dodecyl sulfate (SDS) at a separation temperature and separation voltage of 25°C and 25 kV, respectively. To the author's best knowledge, this is the first CD-MEKC study able to successfully separate the four stereoisomer of vinpocetine in separation time of 9.5 min and resolution of 1.04-3.87.
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