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Updated: May 25, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

RNA aptamer evolution: two decades of SELEction.

Guillermo Aquino-Jarquin¹, Julia D Toscano-Garibay

¹Unit of Research on Oncological Disease, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico. guillaqui@himfg.edu.mx

International Journal of Molecular Sciences

|January 25, 2012

Summary

Aptamers, which are RNA molecules, are selected using the SELEX method. This review traces the evolution of SELEX technology over 20 years for molecular recognition and therapeutic applications.

Keywords:

SELEX aptamers in vitro evolution in vitro selection non-coding RNAs

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Area of Science:

Molecular Biology
Biochemistry
Nucleic Acid Chemistry

Background:

Aptamers are RNA molecules that function similarly to antibodies, offering high specificity and affinity for various targets.
The Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is a key method for aptamer selection.
SELEX technology has been instrumental in identifying structured oligonucleotides for molecular recognition since its inception in 1990.

Purpose of the Study:

To provide a historical overview of the SELEX method.
To highlight the evolution of SELEX over two decades.
To discuss the role of SELEX in developing aptamer-based therapeutics.

Main Methods:

The review focuses on the historical development and applications of the SELEX process.
It examines the adaptation of SELEX with emerging technologies.
The text analyzes the use of SELEX for selecting aptamers against diverse targets, from small molecules to whole cells.

Main Results:

SELEX has proven to be a robust technology for aptamer discovery.
The method has evolved significantly, adapting to new technological advancements.
SELEX has expanded its utility from basic research to the design of therapeutic agents.

Conclusions:

The SELEX method has a rich 20-year history of enabling aptamer selection.
SELEX continues to be a vital tool in molecular medicine and therapeutic development.
The ongoing evolution of SELEX promises further innovation in aptamer-based applications.