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Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
Published on: September 6, 2012
Fragrance release profile from sonochemically prepared protein microsphere containers.
Oshrat Tzhayik1, Artur Cavaco-Paulo, Aharon Gedanken
1Department of Chemistry and Kanbar Laboratory for Nanomaterials, Bar-Ilan University Center for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel.
Ultrasonics Sonochemistry
|January 26, 2012
Summary
Researchers created stable protein microspheres encapsulating fragrant oil with 97% efficiency. These microspheres demonstrate controlled release profiles, offering potential for various applications.
Area of Science:
- Materials Science
- Biotechnology
- Chemical Engineering
Background:
- Protein-based delivery systems are crucial for encapsulating and controlling the release of active compounds.
- Fragrant oils require stable matrices for preservation and controlled volatilization.
Purpose of the Study:
- To develop and characterize protein microspheres for encapsulating amyl acetate (AA).
- To investigate the stability and release profiles of these AA-loaded protein microspheres.
Main Methods:
- Protein microspheres were prepared using sonication of bovine serum albumin (BSA) and amyl acetate (AA).
- Encapsulation efficiency was determined, and microsphere stability was assessed under various storage conditions.
- Release profiles were measured by monitoring evaporation rates at different temperatures.
- Morphological characterization was performed using SEM, ESEM, Cryo-SEM, light microscopy, and confocal laser scanning microscopy.
Main Results:
- Nano- to micrometer-sized protein spheres with approximately 97% encapsulation efficiency for AA were successfully prepared.
- The microspheres exhibited stability for over 6 months at 4°C and 20°C.
- Release profiles showed distinct evaporation rates corresponding to water and AA, with changes linked to container collapse upon water evaporation.
Conclusions:
- Stable, high-efficiency protein microspheres encapsulating amyl acetate can be produced.
- The microspheres exhibit controlled release characteristics influenced by environmental conditions and matrix integrity.
- This method provides a foundation for developing novel delivery systems for volatile compounds.
