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Multiplex Raman induced Kerr effect microscopy
Brandon R Bachler1, Martin E Fermann, Jennifer P Ogilvie
1Department of Physics and Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA.
Optics Express
|January 26, 2012
Summary
We developed a new chemical imaging technique using Raman-induced Kerr effect spectroscopy (RIKES). This method provides spectrally-resolved imaging with reduced background noise, outperforming stimulated Raman scattering (SRS) in certain conditions.
Area of Science:
- Spectroscopy and Chemical Imaging
- Nonlinear Optics
- Microscopy
Background:
- Coherent anti-Stokes Raman scattering (CARS) suffers from nonresonant background noise.
- Raman-induced Kerr effect spectroscopy (RIKES) offers an alternative for spectrally-resolved chemical imaging.
- RIKES can be limited by sample-dependent birefringent background, affecting sensitivity and spectral fidelity.
Purpose of the Study:
- To investigate multiplex RIKES for spectrally-resolved chemical imaging.
- To evaluate the performance of RIKES compared to stimulated Raman scattering (SRS).
- To address the limitations of RIKES, particularly the birefringent background.
Main Methods:
- Utilizing circularly-polarized pump excitation in multiplex RIKES.
- Implementing a high polarization-purity, low frequency chopping scheme.
- Comparing multiplex RIKES microscopy with multiplex SRS microscopy.
Main Results:
- Multiplex RIKES with circular polarization can eliminate the nonresonant background.
- A significant sample-dependent birefringent background was observed in RIKES.
- In low birefringence samples, multiplex RIKES demonstrated an enhanced signal-to-noise ratio over multiplex SRS.
Conclusions:
- Multiplex RIKES is a promising technique for spectrally-resolved chemical imaging.
- Optimized RIKES implementation can overcome background limitations.
- RIKES offers advantages over SRS in specific sample types for chemical imaging applications.
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