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A multiherbal formulation influencing immune response in vitro.
L Menghini1, L Leporini, N Scanu
1Department of Drug Science, University G. D'Annunzio, Chieti, Italy. lmenghini@unich.it
Minerva Medica
|January 27, 2012
Summary
Phytocomplexes from Uncaria, Shiitake, and Ribes modulate immune responses by regulating oxidative stress and cytokine release without affecting cell viability. These natural extracts show potential as immune system regulators.
Area of Science:
- Immunology
- Pharmacology
- Natural Product Chemistry
Background:
- Immune system regulation is crucial for health.
- Phytocomplexes offer potential therapeutic benefits.
- Understanding the impact of natural compounds on immune cells is vital.
Purpose of the Study:
- To evaluate the effects of Uncaria, Shiitake, and Ribes phytocomplexes on immune cell viability and inflammatory responses.
- To assess the impact of these extracts on oxidative stress markers and cytokine gene expression.
Main Methods:
- Standardized extracts and formulations were tested on PBMC, U937, and macrophage cell lines.
- Cell viability was assessed using the MTT assay.
- Oxidative markers (ROS, PGE2) and immune response genes (IL-6, IL-8, TNFα) were analyzed via RT-PCR.
Main Results:
- Extracts did not significantly affect cell viability, even at high doses (up to 500 µg/mL).
- Pre-treatment with extracts reduced basal oxidative marker release and enhanced cellular response to oxidant stimulation.
- Macrophages showed selective immune modulation, with increased IL-6 and IL-8 mRNA and decreased TNFα mRNA.
Conclusions:
- Uncaria, Shiitake, and Ribes extracts act as immune system regulators.
- Mechanisms involve modulation of oxidative stress and selective release of proinflammatory cytokines.
- These findings support the use of these phytocomplexes for immune system support.
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