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Updated: May 25, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Published on: October 15, 2018
Formation of new base pairs between inosine and 5-methyl-2-thiocytidine derivatives
Akihiro Ohkubo1, Yudai Nishino, Yu Ito
1Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan.
Abstract:
In this paper, we report DNA and 2'-OMe-RNA probes containing 5-methyl-2-thiocytidine (m(5)s(2)C) residues that can bind selectively and strongly to the corresponding RNA targets containing inosine residues by the significant stacking effect and steric hindrance of the 2-thiocarbonyl group.
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