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Widespread Emergence of Multidrug Resistant Pseudomonas aeruginosa Isolated from CSF Samples

S Nagaveni, H Rajeshwari, Ajay Kumar Oli

    Indian Journal of Microbiology
    |January 28, 2012
    PubMed

    Insights

    Pseudomonas aeruginosa causes serious central nervous system infections. This study found high rates of multidrug resistance in P. aeruginosa from cerebrospinal fluid samples, posing a significant clinical threat.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Clinical Medicine

    Background:

    • Bacterial meningitis necessitates prompt antibiotic treatment due to rising antimicrobial resistance.
    • Nosocomial infections caused by Pseudomonas aeruginosa are increasing, with a growing trend toward multidrug resistance.
    • Pseudomonas aeruginosa is an opportunistic pathogen that poses a significant threat in healthcare settings.

    Purpose of the Study:

    • To evaluate the antibiotic resistance patterns of Pseudomonas aeruginosa isolates from cerebrospinal fluid (CSF).
    • To determine the prevalence of multidrug resistance and imipenem resistance in P. aeruginosa from CSF samples.

    Main Methods:

    • Analysis of 53 P. aeruginosa isolates obtained from 80 CSF samples.
    • Evaluation of antibiotic resistance against multiple antibiotics.
    • Genotypical characterization using ERIC based PCR.

    Main Results:

    • 80% (42/53) of P. aeruginosa isolates exhibited multidrug resistance to over eight antibiotics.
    • 32% (17/53) of isolates were identified as imipenem-resistant P. aeruginosa (IMPR-Pa).
    • ERIC based PCR indicated minor genetic variations among the isolates, suggesting potential clonal spread or adaptation.

    Conclusions:

    • High prevalence of multidrug-resistant P. aeruginosa in CSF samples highlights a critical challenge in treating central nervous system infections.
    • The increasing resistance, particularly to imipenem, underscores the urgent need for effective therapeutic strategies against P. aeruginosa.
    • Polymicrobial infections are common in CSF, but the high resistance rates of P. aeruginosa make it a significant opportunistic pathogen.
    Keywords:
    AntibioticsCSFMICNosocomial infectionsPrevalencePseudomonas aeruginosa

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