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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

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Updated: May 25, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Proteomics: bases for protein complexity understanding.

Domenico Rotilio1, Anna Della Corte, Marco D'Imperio

  • 1Laboratory of Proteomics and Analytical Techniques, Research Laboratories, Fondazione di Ricerca e Cura Giovanni Paolo II, Catholic University, Campobasso, Italy. domenico.rotilio@rm.unicatt.it

Thrombosis Research
|January 31, 2012
PubMed
Summary

Modern proteomics uses advanced technologies like LC-MS/MS to analyze the complete set of proteins (proteome). This helps understand complex protein functions beyond genomic information, including modifications and interactions.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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Published on: October 19, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
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Published on: June 14, 2017

Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • Genomic sequences do not fully correlate with protein functions due to mRNA splice variants, post-translational modifications (PTM), and moonlighting functions.
  • Studying the entire cell protein population (proteome) requires advanced technologies to handle complexity and wide dynamic ranges.

Purpose of the Study:

  • To provide an overview of current technologies defining and characterizing modern proteomics.
  • To highlight the importance of proteomics in understanding protein functions beyond genomics.

Main Methods:

  • Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) for protein resolution and quantification.
  • Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for high-throughput protein identification, quantification, and characterization.
  • Bioinformatic tools for processing and analyzing large proteomic datasets.

Main Results:

  • Proteomics offers tools for protein identification, quantification, interaction studies, modification analysis, and localization.
  • LC-MS/MS is a sensitive and accurate technique for quantitative proteomic analysis, including PTMs.
  • 2D-PAGE remains a widespread strategy for global protein expression studies.

Conclusions:

  • Comprehensive proteomic analysis is essential in the post-genomic era.
  • Emerging high-throughput technologies are crucial for advancing proteomics.
  • Integrated approaches utilizing various technologies and bioinformatics are key to modern proteomics research.