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Updated: May 25, 2026
Microcrystallography of Protein Crystals and In Cellulo Diffraction
Published on: July 21, 2017
In vivo protein crystallization opens new routes in structural biology
Rudolf Koopmann1, Karolina Cupelli, Lars Redecke
1Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.
Abstract:
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
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