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Correlating matrix metalloproteinase-9 sinus secretion levels with tissue biopsy levels
Matt A Wilson1, Richard R Orlandi, Matthew A Firpo
1Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Utah, Salt Lake City, UT 84132, USA. matthew.wilson@hsc.utah.edu
International Forum of Allergy & Rhinology
|January 31, 2012
Summary
Sinus secretion collection can replace invasive tissue biopsies for measuring matrix metalloproteinase-9 (MMP-9) in chronic rhinosinusitis research. This method reduces patient morbidity and allows for sequential sampling.
Area of Science:
- Otorhinolaryngology
- Biochemistry
Background:
- Nasal mucosal biopsies are invasive and can exacerbate inflammation, hindering the study of sinonasal diseases.
- There is a need for less invasive methods to assess disease markers in chronic rhinosinusitis (CRS).
Purpose of the Study:
- To evaluate if sinus secretions can be used instead of tissue biopsies for protein analysis.
- To determine if MMP-9 levels in secretions correlate with levels in tissue biopsies.
Main Methods:
- Gelatin foam was used to collect ethmoid mucosal secretions before surgical intervention for CRS.
- Tissue biopsies were obtained from the same site for comparison.
- Matrix metalloproteinase-9 (MMP-9) protein levels were quantified in both sample types.
Main Results:
- A significant positive correlation was found between MMP-9 protein levels in secretions and tissue biopsies (p = 0.0033, r = 0.52).
- This indicates that MMP-9 levels are comparable between the two sample types.
Conclusions:
- Sinus secretion collection is a viable, less invasive alternative to tissue biopsy for MMP-9 determination in CRS.
- This method reduces patient morbidity and enables serial sampling from the same anatomical location.
