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Related Concept Videos

Lysosomes01:31

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
Lysosomes01:31

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
Lysosomal Hydrolases01:22

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

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Related Experiment Video

Updated: May 25, 2026

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
05:30

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis

Published on: November 14, 2025

Analyzing lysosomes in live cells.

Paul R Pryor1

  • 1Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York, York, United Kingdom.

Methods in Enzymology
|February 1, 2012
PubMed
Summary
This summary is machine-generated.

Lysosomes are vital organelles for cellular waste removal. New fluorescent probes help researchers measure lysosome function and membrane traffic in live cells, aiding disease research.

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Last Updated: May 25, 2026

Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Lysosomes are crucial cellular organelles involved in degrading macromolecules via multiple pathways.
  • Lysosomal dysfunction is linked to severe human diseases.
  • Over 50 years post-discovery, advanced tools are emerging to study lysosome function.

Purpose of the Study:

  • To describe current methodologies for assessing lysosome function in live cells.
  • To highlight the utility of fluorescent probes in lysosomal research.

Main Methods:

  • Utilizing fluorescent probes to visualize membrane trafficking to lysosomes.
  • Employing probes to assess lysosomal hydrolase activity in real-time.
  • Measuring lysosome function in live cellular models.

Main Results:

  • Established fluorescent probes enable visualization of membrane traffic to lysosomes.
  • Available probes allow for assessment of lysosomal hydrolase activity in live cells.
  • These methods provide insights into lysosome function.

Conclusions:

  • Current methods and fluorescent probes offer powerful tools for studying lysosome function in live cells.
  • Advancements in lysosomal biology research facilitate understanding of disease mechanisms.
  • Further research using these tools can elucidate the role of lysosomes in health and disease.