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Updated: May 25, 2026

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Leukemia inhibitory factor in rat fetal lung development: expression and functional studies.

Cristina Nogueira-Silva1, Paulina Piairo, Emanuel Carvalho-Dias

  • 1Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.

Plos One
|February 1, 2012
PubMed
Summary

Leukemia inhibitory factor (LIF) inhibits fetal rat lung branching by affecting epithelial-mesenchyme signaling. LIF also impacts MAPK, AKT, and STAT3 pathways during lung development.

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Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Respiratory Medicine

Background:

  • Leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) are gp130-type cytokines with shared signal transducers.
  • IL-6 promotes fetal lung branching, and LIF is implicated in other branching organ development.

Purpose of the Study:

  • To investigate the expression pattern of LIF and its receptor LIFRα in fetal rat lungs.
  • To determine the effects of LIF on fetal lung morphogenesis and branching.

Main Methods:

  • Immunohistochemistry and western blot to assess LIF and LIFRα expression.
  • Culture of fetal lung explants with LIF or neutralizing antibodies.
  • Analysis of MAPK, AKT, and STAT3 phosphorylation.

Main Results:

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  • LIF and LIFRα are constitutively expressed in fetal rat lungs, indicating epithelial-mesenchyme cross-talk.
  • LIF supplementation inhibited lung growth and increased p44/42 phosphorylation.
  • LIF inhibition stimulated lung growth via p38 and Akt pathways.

Conclusions:

  • LIF and LIFRα are expressed throughout fetal lung development.
  • LIF plays an inhibitory role in fetal lung branching and morphogenesis.