Related Experiment Videos
Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus
P J Sharp1, B V McInerney, D R Smith
1Biotechnology Australia Pty. Ltd., Roseville, N.S.W.
Journal of Chromatography
|July 20, 1990
Abstract:
Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.