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Simultaneous determination of neutral and amino sugars in biological materials
R J Kraus1, F L Shinnick, J A Marlett
1Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
Journal of Chromatography
|July 27, 1990
Abstract:
A method is described for the simultaneous analysis of nine neutral and three amino sugars. Mixtures of standard sugars and biological samples were acid hydrolyzed with a two step Saeman procedure, neutralized with BaCO3, reduced with sodium borohydride, acidified, evaporated and alditol acetates prepared. Baseline resolution was achieved on a glass-capillary SP-2340 column in ca. 52 min. Reproducibility, response factors and hydrolysis losses were determined. Quantitation was linear over the range of 10-20 micrograms/ml to 2000 micrograms/ml. Conditions were defined for the reproducible quantitation of muramic acid.