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Antibiotic Selection

Antibiotic Selection

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Related Experiment Video

Updated: May 25, 2026

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

In vitro antibacterial resistance selection and quantitation.

Katherine Young¹

¹Merck Research Laboratories, Rahway, New Jersey, USA.

Current Protocols in Pharmacology

|February 2, 2012

Summary

Understanding antibacterial resistance frequency is crucial for developing new drugs. Protocols are provided to determine resistance rates and generate mutants, preventing clinical failure from pre-existing resistant bacteria.

Area of Science:

Microbiology
Pharmacology
Drug Discovery

Background:

Antibacterial resistance poses a significant threat to public health.
The presence of pre-existing resistant mutants can lead to clinical failure of new antibacterial agents.
Determining resistance frequency is vital for the successful development of new antibacterial therapies.

Purpose of the Study:

To provide protocols for determining the frequency and rate of resistance to antibacterial agents.
To outline methods for generating resistant mutants for further study.
To support the discovery and development of novel antibacterial compounds.

Main Methods:

Development of standardized protocols for resistance frequency determination.
Methodologies for isolating and characterizing resistant bacterial mutants.

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Last Updated: May 25, 2026

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

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Published on: December 14, 2019

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Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

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Application of these methods in antibacterial discovery programs.

Main Results:

Established reliable methods for quantifying antibacterial resistance frequency.
Generated characterized resistant mutants for mechanistic studies.
Provided a framework for assessing the clinical viability of new antibacterial agents.

Conclusions:

Accurate determination of resistance frequency is essential for antibacterial drug development.
Protocols presented enable the characterization of resistance mechanisms.
This work aids in the discovery of effective antibacterial agents and prevents clinical failures.